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KMID : 1146920210510030347
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Journal of Pharmaceutical Investigation
2021 Volume.51 No. 3 p.347 ~ p.359
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Characterization studies of novel series of cobalt (II), nickel (II) and copper (II) complexes: DNA binding and antibacterial activity
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Adimule Vinayak
Yallur Basappa C.
Kamat Vinutha
Krishna P. Murali
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Abstract
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Purpose: In the present investigation metal (II) complexes containing binuclear Co (II), mono nuclear Ni (II), and Cu (II) with ligand 2E-N-phenyl-2-[([1,3]thiazolo[3,2-a]benzimidazol-2yl) methylidene]hydrazine-1-carbothioamide moiety were synthesized in ethanolic medium, recrystallized and investigated for their biological properties. The carbothioamide was derived from the reaction of benzo [4, 5] imidazo [2, 1-b] thiazole-2-carbaldehyde and 4-phenyl-3-thiosemicarbazide at reflux temperature.
Methods: The newly formed compounds were characterized by 1H-nuclear magnetic resonance (1H-NMR), 13C-nuclear magnetic resonance (13C-NMR), Fourier transformed infrared spectroscopy (FT-IR), ESR spectroscopic techniques and elemental analysis. Ligand containing “O” and “S” donor atoms participated in coordination with metal (II) complexes and are confirmed by FT-IR, electronic absorption spectra, 1H-NMR data. DNA binding properties of these metal complexes were investigated by ESR, magnetic moments and electronic absorption spectroscopy.
Results: Antibacterial studies, DNA binding constants of the complexes indicated intercalative binding mode involving strong aromatic chromophoric groups and base pairs of DNA. All the complexes (2a, 2b and 2c) showed intercalative binding with DNA and DNA cleavage was studied in presence and absence of H2O2 which was observed for copper (II) complex.
Conclusion: Antibacterial study results showed that cobalt complex inhibit the growth of Staphylococcus epidermis, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli with MIC values of 7.98 μg/mL, 8.12 μg/mL, 7.34 μg/mL, and 7.56 μg/mL respectively. Metal (II) complexes exhibit higher antibacterial activity than the parent ligand.
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KEYWORD
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2-Mecapatobenzimidazole, DNA cleavage, DNA binding activity, Antibacterial, Transition metal complexes
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